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Application of rapid lysis techniques in radiobioloy. III. The effect of radiosensitizers on the production of DNA damage and the time course of its repair. [Escherichia coli, para-nitroacetophenone, norpseudopelletierine-N-oxyl, gamma radiation]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574868· OSTI ID:6952205
; ;

Para-nitroacetophenone (PNAP) increases the yield of single-strand breaks (ssb) measured 0.2 sec after irradiation of Escherichia coli B/r to the same extent as does oxygen. Norpseudopelletierine-N-oxyl (NPPN) does not modify the yield of ssb seen. By studying the time course of the enzymatic repair of ssb and DNA ''fixed damage'' with E. coli B/r and K/sub 12/ Pol A/sup -//sub 1/ it was shown that neither PNAP or NPPN enhanced the yield of fixed damage of the type produced by oxygen. Repair of radiation-produced ssb in the NPPN experiments was inhibited so that after approximately 120 sec the yield of ssb was the same as was found with PNAP or O/sub 2/. It was concluded that PNAP can only mimic one of the effects of oxygen on DNA damage and that NPPN modifies DNA damage in a different manner to oxygen.

Research Organization:
Inst. of Cancer Research, Sutton, Eng.
OSTI ID:
6952205
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 75:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
IONIZING RADIATIONS
KETONES
LYSIS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOBIOLOGY
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
STRAND BREAKS
TIME DEPENDENCE