Anoxic radiosensitization of two Bacillus species by p-nitroacetophenone
P-nitroacetophenone (PNAP) has been tested for its ability to alter radiation sensitivity in two closely related bacilli. PNAP is an anoxic sensitizer of spores and vegetative cells of both B. megaterium and B. pumilus, although the extent of PNAP's sensitization differs in each of the four test systems. In the spores, PNAP increased the anoxic responses by 40% in B. megaterium and 20% in B. pumilus. (The oxygen enhancement ratios here are 2.14 and 2.22, respectively.) In both vegetative cells, PNAP achieved full oxygen- level sensitization, increasing the anoxic responses of B. megaterium by l30% and B. pumilus by 60%. These factor-of-two ratios in PNAP's efficiency in both the spores and vegetative cells suggest this difference might be due to inherent differences in the cells themselves. The ratios of the amounts of DNA in the spores and vegetative cells correspond closely to the ratios of PNAP's maximum efficiencies in these cells. Those results, while not identifying DNA as the target, suggest that specific reactions between PNAP and possible target molecules should be studied. (auth)
- Research Organization:
- Inst. of Cancer Research, Sutton, Eng.
- NSA Number:
- NSA-29-015907
- OSTI ID:
- 4357912
- Journal Information:
- Int. J. Radiat. Biol., v. 24, no. 5, pp. 505-515, Journal Name: Int. J. Radiat. Biol., v. 24, no. 5, pp. 505-515; ISSN IJRBA
- Country of Publication:
- United Kingdom
- Language:
- English
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Related Subjects
*BACILLUS PUMILIS-- RADIOSENSITIVITY
*BACILLUS-- RADIOSENSITIVITY
*KETONES-- RADIOSENSITIVITY EFFECTS
*NITRO COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*OXYGEN-- RADIOSENSITIVITY EFFECTS
ANOXIA
DNA
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
OXYGEN ENHANCEMENT RATIO
RADIOSENSITIZERS
RESPONSE MODIFYING FACTORS
SPORES