Influence of the radiosensitizers paranitroacetophenone and triacetoneamine- N-oxyl on the effects of ionizing radiation on DNA

Journal Article · Sat Dec 01 04:00:00 EST 1973 · Int. J. Radiat. Biol., v. 24, no. 6, pp. 569-580

DOI:https://doi.org/10.1080/09553007314551481· OSTI ID:4345693

Van Hemmen, J J; Meuling, W J.A.; Bleichrodt, J F

Paranitroacetophenone (PNAP) and triacetoneamine-N-oxyl (TAN), which sensitize anoxic cells to ionizing radiation, did not increase the radiosensitivity of bacteriophage DNA irradiated in the dry state. It is suggested that this shows the direct action model' of sensitization does not hold in this experimental set-up. PNAP and TAN added within 1.5 msec after irradiation of the DNA in buffer, did not decrease the survival of biological activity, indicating that any reaction between these compounds and longlived' DNA radicals is not lethal. When DNA was irradiated in solution in the presence of TAN, part of the damage in DNA was reparable by the excision repair system of Escherichia coli. Such an effect was not observed with PNAP. In protective media PNAP and TAN also behaved differently with regard to the inactivation of phage T/sub 1/. Like oxygen, PNAP protected the phage a bit and decreased the difference in survival on a Uvr/sup +/ and a Uvr/sup -/ strain of E. coli. TAN sensitized T/sub 1/ and increased the difference on the two strains. Induction of mutations in phage by radiation, which is often enhanced by O/sub 2/, was not significantly affected by the presence of the sensitizers. (auth)

• Research Organization: Rijksverdedigingsorganisatie TNO, Rijswijk, Netherlands
• NSA Number: NSA-29-018557
• OSTI ID: 4345693
• Journal Information: Int. J. Radiat. Biol., v. 24, no. 6, pp. 569-580, Journal Name: Int. J. Radiat. Biol., v. 24, no. 6, pp. 569-580; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*BACTERIOPHAGES-- MUTATION FREQUENCY
*DNA-- RADIOSENSITIVITY
*KETONES-- RADIOSENSITIVITY EFFECTS
*ORGANIC NITROGEN COMPOUNDS-- RADIOSENSITIVITY EFFECTS
ANOXIA
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
BUFFERS
ESCHERICHIA COLI
GAMMA RADIATION
IONIZING RADIATIONS
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
OXYGEN
PHOTON BEAMS
RADICALS
RADIOSENSITIZERS
RESPONSE MODIFYING FACTORS
SOLUTIONS
SURVIVAL CURVES
TIME DEPENDENCE
WATER