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Application of rapid lysis techniques in radiobiology. I. The effect of oxygen and radiosensitizers on DNA strand break production and repair in E. coli B/r

Journal Article · · Radiat. Res., v. 64, no. 3, pp. 431-442
DOI:https://doi.org/10.2307/3574233· OSTI ID:4102298
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The number of single-strand breaks per single-strand genome produced by irradiating E. coli B/r in suspension has been measured as a function of elapsed time between irradiation and lysis. Between 0.2 and 2 sec after irradiation, the number of breaks in O$sub 2$ and N$sub 2$ remains constant at 32 and 9, respectively, following a 32 krad dose delivered in 12 msec. These values are the same at all temperatures between 0$sup 0$C and 37$sup 0$C. After 2 sec both excision and strand rejoining processes are observed with temperature-dependent rates. The oxygen enhancement ratio for ssb measured 300 sec after irradiation is 5.2 compared with 3.6 at 0.2 sec and 3.0 for cell survival. The radiosensitizer PNAP mimics oxygen in producing single-strand breaks, whereas NPPN does not affect the number of breaks observed in N$sub 2$ but it does inhibit repair. (auth)

Research Organization:
Inst. of Cancer Research, Sutton, Eng.
Sponsoring Organization:
USDOE
NSA Number:
NSA-33-015026
OSTI ID:
4102298
Journal Information:
Radiat. Res., v. 64, no. 3, pp. 431-442, Journal Name: Radiat. Res., v. 64, no. 3, pp. 431-442; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

*BIOLOGICAL REPAIR-- TEMPERATURE DEPENDENCE
*DNA-- STRAND BREAKS
*OXYGEN-- RADIOSENSITIVITY EFFECTS
*RADIOSENSITIZERS-- RADIOSENSITIVITY EFFECTS
*STRAND BREAKS-- RADIOINDUCTION
560112* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Microorganisms
BIOLOGICAL RADIATION EFFECTS
ELECTRONS
ESCHERICHIA COLI
IRRADIATION
N48120* --Life Sciences--Radiation Effects on Biochemicals-- In Microorganisms
N48310 --Life Sciences--Radiation Effects on Microorganisms--Basic Studies