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Application of rapid-lysis techniques in radiobiology. IV. The effect of glycerol and DMSO on Chinese hamster cell survival and DNA single-strand break production. [/sup 60/Co]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575466· OSTI ID:6460413
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A rapid-lysis technique has been used to compare the initial yield of DNA single-strand breaks (ssb) measured 0.2 sec after irradiation and cell survival for Chinese hamster cells in vitro. Both DMSO and glycerol protected cells irradiated in air, and there was a simple relationship between relative radiosensitivity and the initial number of ssb. Under oxic conditions at high concentrations (2 M) of either agent the yield of ssb was reduced by a factor of 3 and the slope of the survival curve by a factor of 2. Under hypoxic conditions much less protection was noted, and even at high concentrations of either agent (2 M) there was only a small degree of protection against cell inactivation and ssb.

Research Organization:
Inst. of Cancer Research, Surrey, England
OSTI ID:
6460413
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 86:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DMSO
DNA
EARLY RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
GLYCEROL
HAMSTERS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LYSIS
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
STRAND BREAKS
SULFOXIDES
SURVIVAL CURVES
VERTEBRATES
YEARS LIVING RADIOISOTOPES