Intestinal uptake of fluorescent microspheres in young and aged mice (42825)

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

OSTI ID:6937876

LeFevre, M E; Boccio, A M; Joel, D D ^[1]

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1. Brookhaven National Laboratory, Upton, NY (USA)

Rhodamine B-labeled synthetic latex particles (microspheres), 1.8 {mu}m in diameter, were administered by gavage 5 days per week to young (24 days) and aged (18 months) mice. After 25 days (19 gavages), the particles were assayed in solubilized tissues by depositing them of filters and counting under fluorescence microscopy. Aged mice exhibited significantly more fluorescent particle accumulation in Peyer's patches but significantly less in lungs than young mice. Mesenteric lymph nodes and Peyer's patch-free intestinal segments contained measurable latex, but differences between young and aged animals were not significant. Liver contained only trace amounts of latex, and spleen and kidney were latex free in both young and aged animals. Nonquantitative observations on KOH-glycerol-cleared whole Peyer's patches and slices of liver, lung, and mesenteric lymph node were similar.

• OSTI ID: 6937876
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Journal Name: Proceedings of the Society for Experimental Biology and Medicine; (USA) Vol. 190:1; ISSN PSEBA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AGE DEPENDENCE
ANIMALS
BODY
DIGESTIVE SYSTEM
DISTRIBUTION
FLUORESCENCE
GLANDS
INTESTINAL ABSORPTION
KIDNEYS
LIVER
LUMINESCENCE
LUNGS
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MICE
MICROSCOPY
MICROSPHERES
ORGANS
PHYSIOLOGY
RESPIRATORY SYSTEM
RODENTS
SPLEEN
TISSUE DISTRIBUTION
UPTAKE
VERTEBRATES