Intestinal barrier to large particulates in mice

Journal Article · Tue Jan 01 00:00:00 EST 1980 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398009529888· OSTI ID:6288892

LeFevre, M E; Hancock, D C; Joel, D D

Intestinal barrier function in mice was assessed after acute or chronic oral administration of 15.8- and 5.7-..mu..m synthetic spherical particles. The results failed to confirm previous reports that ingested particles rapidly appear in blood. Furthermore, 15.8-..mu..m particles did not accumulate in intestinal Peyer's patches, mesenteric lymph nodes, or other organs of the reticuloendothelial system, even after the maximum dosage of 8 x 10/sup 6/ particles per day for 60 d. However, the 5.7-..mu..m particles were demonstrated in Peyer's patches, mesenteric lymph nodes, and lungs after the maximum dosage of 4.5 x 10/sup 5/ particles per day for 60 d. At 77 d after the termination of ingestion, 5.7-..mu..m particles were still present in these tissues. The 5.7-..mu..m particles were not found in spleen; retention in liver was equivocal. The site of uptake of particles capable of penetrating the intestinal mucosa appears to be the Peyer's patches. It is suggested that most absorbed particles are sequestered in Peyer's patch macrophages. Particles that escape sequestration are transported by lymph rather than by portal blood. The findings indicate that hazards associated with intestinal uptake of large (> 5 ..mu..m) particulates exist, but that the frequency of such penetration is still unclear.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6288892
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INTESTINES
DYNAMIC FUNCTION STUDIES
PARTICLES
RETENTION FUNCTIONS
BLOOD
BUILDUP
LYMPH NODES
METABOLISM
MICE
MUCOUS MEMBRANES
PARTICLE SIZE
PHYSIOLOGY
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RETENTION
UPTAKE
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
FUNCTIONS
GASTROINTESTINAL TRACT
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MEMBRANES
ORGANS
RODENTS
SIZE
VERTEBRATES
551000* - Physiological Systems