Presentation of anti-idiotypic antibody is sensitive to ionizing radiation

Journal Article · Fri Jul 15 04:00:00 EDT 1994 · Journal of Immunology; (United States)

OSTI ID:6936675

Johnson-Leger, C A; Dean, C J ^[1]

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1. Institute of Cancer Research, Sutton (United Kingdom)

The authors have been investigating the generation of specific immune responses using monoclonal anti-idiotypic Abs (Ab2) as surrogate tumor Ag. They have prepared a series of idiotypic mAbs (Ab1) from CBH/cbi rats bearing the syngenic sarcoma HSN and have used these Ab1 to generate autologous Ab2. By using the autologous Ab2 as Ag, they have isolated T cell lines from CBH/cbi rats that proliferate specifically in the presence of the Ab2, with spleen cells as APC. Specific proliferation of the T cells was prevented if the spleen cells used for presentation were irradiated with conventional doses of x-rays (1000 rad) just before use. Titration of the radiation response showed that the capacity of the spleen cells to present Ag decreased exponentially with x-ray doses of up to 100 rad, at which dose presentation was virtually abolished. The same irradiated spleen cells were fully competent to present OVA to CBH/cbi-derived rat T cell lines specific for this Ag. Preincubating the APC with Ag before irradiation abrogated the effect of x-irradiation on the presentation of Ab2. The authors conclude that, in this rat system, the presentation of autologous Ab2 is highly sensitive to the effects of low doses of x-rays. The clinical significance of these findings is discussed. 48 refs., 8 figs., 3 tabs.

• OSTI ID: 6936675
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 153:2; ISSN 0022-1767; ISSN JOIMA3
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
SPLEEN CELLS
X RADIATION