Studies of guinea pig immunoglobulin isotype, idiotype and antiidiotype
Thesis/Dissertation
·
OSTI ID:7147266
Immunization of Guinea pigs with diphtheria toxoid generated antibodies of the IgG class that were capable of neutralizing native toxin in vivo. Sera from these animals were used to affinity purify idiotypic antibodies (AB1). AB1 vaccines derived from the IgG1 class and from F(ab{prime}){sub 2} of IgG1 + IgG2 (IgG1/2) classes were effective in inducing a syngeneic anti-idiotype (AB2) response. Animals immunized with AB1 consisting of both IgG1/2 did not elicit a detectable AB2 response. Binding of homologous {sup 125}I-F(ab{prime}){sub 2} (AB1) to the antiidiotype was inhibited 90% in the presence of DT.F(ab{prime}){sub 2} derived from preimmune serum or had no inhibitory effects on the idiotype-antiidiotype interactions. Two groups of outbred guinea pigs were vaccinated with alum absorbed F(ab{prime}){sub 2} of anti-idiotype IgG1/2 (AB2). Of the ten animals inoculated with AB2, three tested positive by RIA against {sup 125}I-DT. Two of the RIA positive sera contained antibodies that neutralized diphtheria toxin in a rabbit intracutaneous assay. Purification of guinea pig IgG by protein A-Sepharose affinity chromatography resulted in the separation of three distinct IgG populations.
- Research Organization:
- Massachusetts Univ., Amherst, MA (USA)
- OSTI ID:
- 7147266
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOSYNTHESIS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GUINEA PIGS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VACCINES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOSYNTHESIS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GUINEA PIGS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VACCINES
VERTEBRATES