Auto-anti-idiotypic approach to adenosine receptors

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5389009

Ku, H H; Erlanger, B F

If an antibody (Abl) binds to and recognizes the same epitopes on a ligand that are recognized by a receptor, then among anti-idiotypic antibodies (Ab2) to Ab1, there may be some that have combining sites that mimic the ligand (internal image). In the case of the acetylcholine receptor and the glucocorticoid receptor these Ab2 antibodies could be raised through a naturally occurring idiotypic network by immunizing mice with the ligands of the receptors. To test the generality of this procedure and to raise antibodies to adenosine receptors, BALB/c mice were immunized with adenosine 6-aminocaproyl-BSA. Hyberidoma cell lines were raised. Cell lines which secreted antibodies that bound to rabbit anti-adenosine antibody were obtained. Two such mAbs, AA18 and AA21, inhibited the binding of (/sup 3/H)adenosine to rabbit anti-adenosine antibody. Furthermore, they bound to rat cerebral cortical membrane and binding could be inhibited by L-phenylisopropyladenosine (L-PIA), an adenosine receptor agonist. Rat cerebral membrane proteins were solubilized with 1% cholic acid, and analyzed by SDS-PAGE and Western blotting. Both AA18 and AA21 recognized a 62 kD band under non-reducing conditions, and 52 kD and 36 kD bands under reducing conditions. The 36 kD band is consistent with the A1 adenosine receptor binding subunit reported by Stiles, et al. The authors results extend the utility of the autoimmune approach for raising antibody to receptors without the necessity of using isolated receptor as immunogen.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5389009
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
HYBRIDOMAS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MICE
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
REACTION KINETICS
RECEPTORS
RIBOSIDES
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES