Idiotypic cascades in cancer patients treated with monoclonal antibody CO17-1A

Journal Article · Mon May 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.10.3787· OSTI ID:5442354

Wettendorff, M; Iliopoulos, D; Tempero, M; Kay, D; DeFreitas, E; Koprowski, H; Herlyn, D ^[1]

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1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)

The authors have previously shown that gastrointestinal cancer patients treated with monoclonal antibody CO17-1A (Ab1) developed anti-idiotypic antibodies (Ab2) to the Ab1. Radioimmunoassay was used to measure the inhibition by Ab2. They now demonstrate that patients produce anti-anti-idiotypic antibodies (Ab3) to their autologous Ab2. Ab3 were demonstrated in culture supernatants of peripheral blood mononuclear cells from five Ab1-treated patients after stimulation of the cells with heterologous Ab2 that functionally mimicked the tumor antigen (Ag) defined by Ab1 and immunologically cross reacted with the patients' Ab2. Ab3 shared idiotopes with Ab1 and were Ab1-like in their binding specificities to tumor cells, Ag, and Ab2. Such antibodies were also elicited by stimulating cells with Ag. However, they were not produced by stimulating posttreatment mononuclear cells with control proteins or by stimulating pretreatment cells with either Ag or Ab2. The results demonstrate idiotypic cascades in cancer patients treated with monoclonal antibody. Ag-specific Ab3 responses may underlie delayed clinical responses often observed in cancer patients treated with monoclonal antibodies of various specificities.

• OSTI ID: 5442354
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:10; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
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BIOASSAY
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
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IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
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IODINE ISOTOPES
ISOTOPE APPLICATIONS
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METASTASES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES