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Spontaneous and mitogen-induced proliferative activity of mononuclear cells in patients with pollinosis

Journal Article · · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
OSTI ID:6934995
The induction of the proliferative response of B cells by lipopolysaccharide (LPS) and the proliferative activity of T lymphocytes during stimulation by phytohemagglutinin (PHA) in patients with pollinosis, with hypersensitivity to timothy-grass pollen, were studied. Peripheral blood mononuclear cells (MNC) from 17 patients with pollinosis aged from 16 to 40 years, were used. Lymphocytes (MNC) were isolated form heparinized blood by centrifugation and LPS from E. coli serotype 026:B6 in final concentrations was used as B-cell activator. Tritium-thymidine was added 18 h before the end of the culture time. Incorporated radioactivity was counted in a Mark III liquid scintillation beta-counter. The hyperproduction of IgE observed in atopic patients is more likely to be connected with changes in the later stages of differentiation of IgE-synthesizing B-lymphocytes (at the memory cell level) into IgE-producing plasma cells.
Research Organization:
Institute of Immunology, Ministry of Health
OSTI ID:
6934995
Journal Information:
Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 99:1; ISSN BEXBA
Country of Publication:
United States
Language:
English

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Related Subjects

550604* -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
AGGLUTININS
ALLERGY
ANIMAL CELLS
ANTIBODIES
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DOSE-RESPONSE RELATIONSHIPS
GAMETES
GERM CELLS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PATIENTS
PHYTOHEMAGGLUTININ
POLLEN
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RADIOISOTOPE SCANNING
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
RIBOSIDES
SACCHARIDES
SCINTISCANNING
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS