Mitogen induced proliferative responses of lymphocytes from spot (Leiostomus xanthurus) exposed to polycyclic aromatic hydrocarbon contaminated environments

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Immunopharmacology and Immunotoxicology; (United States)

DOI:https://doi.org/10.3109/08923979109019707· OSTI ID:6101377

Faisal, M; Marzouk, M S; Smith, C L; Huggett, R J ^[1]

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1. Virginia Institute of Marine Science, School of Marine Science, College of William Mary, Gloucester Point (United States)

The marine fish spot, Leiostomus xanthurus, was collected from five sites in the lower Chesapeake Bay system representing a gradient of sediment polycyclic aromatic hydrocarbon (PAH) concentrations. The proliferative responses to mitogens by anterior kidney lymphocytes were assessed using (3H)-thymidine uptake by replicating DNA. The data show two different mitogen-dependent lymphocytic responses as the sediment PAH levels increase at the sampling sites; a suppression of the response to the T cell mitogens, concanavalin A (Con A) and phytohemagglutinin, and a sharp augmentation of the response to B cell mitogen, lipopolysaccharide (LPS), as well as to poke weed mitogen and peanut agglutinin. The magnitude of the lymphoproliferative responses correlated strongly with the total sediment PAH concentrations (r2 greater than 0.8). A similar correlation was also observed with 15 selected individual PAH compounds regardless of their molecular weights. By maintaining the fish in clean York River water for up to 24 weeks, it was possible to reverse the augmented proliferative responses to LPS of fish from all sampling sites and to increase the reduced responses to Con A, in fish from three sites, and partially in two sites where sediments were highly contaminated with PAH. These results suggest that the proliferative responses of fish lymphocytes to mitogens may be a potentially sensitive biomarker of exposure to, and effects of xenobiotics.

• OSTI ID: 6101377
• Journal Information: Immunopharmacology and Immunotoxicology; (United States), Journal Name: Immunopharmacology and Immunotoxicology; (United States) Vol. 13:3; ISSN 0892-3973; ISSN IITOE
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550501 -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
AROMATICS
ATLANTIC OCEAN
AZINES
BAYS
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL PROLIFERATION
CHESAPEAKE BAY
COASTAL WATERS
CONCANAVALIN A
CONNECTIVE TISSUE CELLS
FISHES
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LECTINS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SEAS
SENSITIVITY
SOMATIC CELLS
SURFACE WATERS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER POLLUTION