Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2/sup +/ and Mn/sup 2+/ on the mitogenic response of carp pronephros lymphocytes

Journal Article · Fri Mar 31 23:00:00 EST 1989 · Ecotoxicol. Environ. Saf.; (United States)

DOI:https://doi.org/10.1016/0147-6513(89)90037-7· OSTI ID:5695797

Ghanmi, Z; Rouabhia, M; Othmane, O; Deschaux, P A

Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn/sup 2+/ (10(-7) to 10(-3) M) to mitogen-stimulated T and B cells enhanced (/sup 3/H)thymidine incorporation. Addition of 10(-5) M Zn/sup 2+/ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10(-7) to 10(-3) M) to mitogen-stimulated lymphocytes enhanced (/sup 3/H)thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10(-1) M Mn/sup 2+/ to lymphocyte cultures exerted an inhibitor on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10(-1) M Mn/sup 2+/ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn/sup 2+/ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10(-1) M Mn2+ could be reversed by adding 2 mM CaCl/sub 2/ to the culture.

• Research Organization: Laboratoire d'Immunophysiologie generale et comparee, Limoges (France)
• OSTI ID: 5695797
• Journal Information: Ecotoxicol. Environ. Saf.; (United States), Journal Name: Ecotoxicol. Environ. Saf.; (United States) Vol. 17:2; ISSN EESAD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ALKALINE EARTH METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CARBOHYDRATES
CATIONS
CELL DIVISION
CELL PROLIFERATION
CHARGED PARTICLES
CONCANAVALIN
CONNECTIVE TISSUE CELLS
ELEMENTS
FISHES
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IN VITRO
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LECTINS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MAGNESIUM COMPOUNDS
MATERIALS
METALS
MITOGENS
MITOSIS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLLUTION
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER POLLUTION
ZINC COMPOUNDS