Phorbol myristate acetate stimulates RNA and casein synthesis in cultured mouse mammary gland tissues

Journal Article · Thu May 01 00:00:00 EDT 1986 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-182-42302· OSTI ID:6933211

Rillema, J A; Waters, S B

Prolactin and phorbol myristate acetate (TPA) stimulate the rate of (/sup 3/H)uridine incorporation in cultured mouse mammary gland explants in a similar fashion. Both the time-courses and magnitude of responses were the same; in addition, maximum stimulatory concentrations of TPA and prolactin elicited a nonadditive response when tested together. Nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, abolished both the TPA and prolactin effects on (/sup 3/H)uridine incorporation. TPA also effected an enhanced rate of (/sup 3/H)leucine incorporation into a casein-rich phosphoprotein fraction, but only if the explants were also cultured with spermidine.

• Research Organization: Wayne State Univ., Detroit, MI
• OSTI ID: 6933211
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 182:1; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CARCINOGENS
CASEIN
ENZYME INHIBITORS
ESTERS
GLANDS
GONADOTROPINS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LTH
MAMMALS
MAMMARY GLANDS
MICE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHORBOL ESTERS
PITUITARY HORMONES
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
STIMULATION
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE
VERTEBRATES