Effects of prolactin on lipid biosynthesis and protein kinase C in mouse mammary gland and NB sub 2 node lymphoma cells
In cultured mouse mammary gland explants derived from 12-14 day pregnant mice, prolactin (PRL) stimulates an increased rate of incorporation of ({sup 14}C)acetate and ({sup 3}H)glucose into triglycerides. The effect is significant between 4-6 hours after addition of PRL. Enzymes likely to be rate-limiting to this process include acetyl CoA carboxylase (ACC), fatty acid synthetase, acetyl CoA synthetase, and/or pyruvate dehydrogenase (PDH). It is possible that early perturbations of phospholipid (PL) metabolism may represent the initial cellular effects of PRL. Consequently the effect of PRL on the incorporation of several precursors into PLs was determined. Employing ({sup 14}C)acetate as a substrate, PRL stimulates its incorporation into phosphatidylcholine, as early as 1-2 hours, and phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine by 2-4 hours. ({sup 3}H)Glycerol incorporation into triglycerides was significantly enhanced by PRL between 4-6 hours, but not into PLs until after 16 hours. Similarly, PRL did not enhance incorporation of ({sup 32}P)O{sub 4}, ({sup 3}H)choline, ({sup 3}H)inositol or ({sup 3}H)seine into PLs until 14-16 hours after addition to culture. 12-O-tetradeconyl-phorbol-13-acetate (TPA) was found to increase ({sup 3}H)uridine incorporation into RNA, and ({sup 3}H)leucine incorporation into caseins in a PRL-like manner. In addition, PRL stimulates a transient, time-dependent translocation of PKC to the particulate fraction of mammary gland explants.
- Research Organization:
- Wayne State Univ., Detroit, MI (USA)
- OSTI ID:
- 6528660
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ALDEHYDES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARCINOGENS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
GLUCOSE
GONADOTROPINS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROXY ACIDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
LIPIDS
LTH
LYASES
MAMMALS
MAMMARY GLANDS
MICE
MONOSACCHARIDES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
RADIOISOTOPES
REACTION KINETICS
RNA
RODENTS
SACCHARIDES
SERINE
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRIGLYCERIDES
TRITIUM COMPOUNDS
VERTEBRATES