Effect of prolactin on enzymes of lipid biosynthesis in mammary gland explants

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6037196

Waters, S B; Rillema, J A ^[1]

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1. Wayne State Univ. School of Medicine, Detroit, MI (USA)

Prolactin (PRL) stimulates an increased rate of incorporation of ({sup 14}C)acetate and ({sup 3}H)glucose into lipids in cultured mammary gland explants from 10-to 14-day-pregnant mice. This response is biphasic with an early increase occurring from 6 through 12 h, and an additional increase from 16 to 24 h. Enzymes likely to be rate limiting to this process include acetyl CoA carboxylase, fatty acid synthetase, acetyl CoA synthetase, and/or pyruvate dehydrogenase. Of these enzymes only pyruvate dehydrogenase activity was elevated at 6 h, suggesting that this enzymatic activity is important in stimulating early increases in lipogenesis after PRL treatment. In addition, the PRL stimulation of pyruvate dehydrogenase may also indirectly stimulate acetyl CoA carboxylase through the generation of citrate; this may explain the early (6-12 h) effect of PRL on ({sup 14}C)acetate incorporation. After 16 h of PRL treatment, the activities of all the lipogenic enzymes were enhanced. The second phase of PRLs stimulation of lipogenesis thus likely involves the enhanced activities of more than one of the lipogenic enzymes.

• OSTI ID: 6037196
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:4; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID ESTERS
ALDEHYDES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CARBOXYLIC ACID ESTERS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
GLUCOSE
GONADOTROPINS
HEMIACETAL DEHYDROGENASES
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
LABELLED COMPOUNDS
LIGASES
LIPIDS
LTH
LYASES
MAMMALS
MAMMARY GLANDS
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
RODENTS
SACCHARIDES
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES