Role of phospholipids in the actions of prolactin in the mammary gland
Thesis/Dissertation
·
OSTI ID:6411904
These studies were designed to determine the role of phospholipid turnover in the mechanism of action of prolactin in mammary gland explants derived from 12-14 day pregnant mice. Prolactin stimulates phospholipid biosynthesis 12-16h after cultured mouse mammary tissues are exposed to it. Prolactin stimulates phospholipid biosynthesis at physiological concentrations and the response is maximal at all PRL concentrations above 25 ng/ml. p-Bromphenacyl bromide (BPB) at concentrations of 50 ..mu..M and above and quinacrine (50 ..mu..M) abolish the actions of prolactin on casein and lipid biosynthesis in cultured mouse mammary gland explants. In mouse mammary gland explants, binding of prolactin to its receptor leads to a phospholipase C type hydrolysis of inositol phospholipids, but this effect is transient and does not occur immediately after hormone exposure. Prolactin significantly stimulated the accumulation of (/sup 3/H)label in inositol monophosphate (IP/sub 1/), inositol bisphosphate (IP/sub 2/) and inositol trisphosphate (IP/sub 3/) 1-3 hours after addition of prolactin. Gossypol, a drug which has been shown to be an inhibitor of kinase C activity in mouse mammary tissues, is shown to abolish several of the actions of prolactin in cultured mouse mammary gland expalants.
- Research Organization:
- Wayne State Univ., Detroit, MI (USA)
- OSTI ID:
- 6411904
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CARBOXYLESTERASES
CHEMISTRY
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
FUNCTIONS
GLANDS
GONADOTROPINS
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPASE
LIPIDS
LTH
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
PREGNANCY
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CARBOXYLESTERASES
CHEMISTRY
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
FUNCTIONS
GLANDS
GONADOTROPINS
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPASE
LIPIDS
LTH
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
PREGNANCY
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES