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Measurement by radioimmunoassay of casein content in rabbit mammary gland during pregnancy and after prolactin stimulation in organ culture

Journal Article · · Proc. Soc. Exp. Biol. Med.; (United States)
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A specific homologous radioimmunoassay was developed to measure rabbit ..beta..-casein in rabbit mammary gland with a sensitivity of 0.5 ng/ml protein. It was used to measure casein concentration during pregnancy and in organ culture of mammary gland explants. Casein was detectable in virgin mammary glands, showed a small increase during the first half of pregnancy, increased more than 20-fold between Days 21 and 27, and diminished somewhat on the first days of lactation. After 24 hr of culture, mammary gland explants had no detectable casein, but the addition of increasing concentrations of prolactin to a culture medium which contained insulin (5 ..mu..g/ml) and cortisol (0.5 ..mu..g/ml) induced a regular increase in the casein content of the tissue. Casein started to increase when 10 ng/ml of prolactin was present and maximal values were achieved for 100 ng/ml of the hormone.

Research Organization:
Institut National de la Recherche Agronomique, Jouy-en-Josas, France
OSTI ID:
6928942
Journal Information:
Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 184:1; ISSN PSEBA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CASEIN
CORTICOSTEROIDS
GLANDS
GLUCOCORTICOIDS
GONADOTROPINS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INSULIN
ISOTOPE APPLICATIONS
KETONES
KINETICS
LTH
MAMMALS
MAMMARY GLANDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANCY
PREGNANES
PROTEINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES