Role of protein kinase C in lymphokine growth factor regulation of oncogene transcription

Journal Article · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6914028

Birchenall-Sparks, M C; Farrar, W L; Rennick, D; Ruscetti, F W

Transcriptional activation of the cellular proto oncogenes (c-onc), c-fos, c-myc, and c-myb, in response to phorbol myristate acetate (PMA), interleukin 2 (IL 2) and interleukin 3 (IL 3) was studied in responsive cell lines by nuclear run off transcription assays. Both recombinant human IL 2 which stimulates the murine T lymphocyte clone CT6, and recombinant Cos 7-derived IL 3 which stimulates the murine myeloid FDC-P1 line both induced a sequential increase in c-fos, c-myc, and c-myb mRNA synthesis. It has been also shown that both IL 2 and 3 stimulate the activation of protein kinase C (PK-C) in their respective interleukin dependent cell lines. PMA, a direct activator of PK-C, also resulted in a similar temporal induction in mRNA synthesis for the same c-onc genes. An increase in the expression of the c-fos, c-myc, and c-myb mRNAs was confirmed by Northern blot analysis. These results indicate that PMA and lymphokine growth factors regulate early c-onc gene transcription by similar biochemical pathways. Since both phorbol ester and interleukins activate PK-C, this phosphotransferase system appears to be an important pathway in gene activation by these agents. The data suggest that these structurally unrelated growth factors promote growth in cells of distinct histologic origins by similar mechanisms involving PK-C activation.

• Research Organization: Lab. of Molecular Immunoregulation, Frederick, MD
• OSTI ID: 6914028
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CARCINOGENS
ENZYME ACTIVITY
ENZYMES
ESTERS
GENES
GROWTH FACTORS
LYMPHOKINES
MITOGENS
ONCOGENES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
TRANSCRIPTION
TRANSFERASES