Glucocorticoids selectively inhibit the transcription of the interleukin 1. beta. gene and decrease the stability of interleukin 1. beta. mRNA
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Transcription of the interleukin 1..beta.. (IL-1..beta..) gene was studied by mRNA hybridization with a cDNA probe in the human promonocytic cell line U-937. Phorbol ester and lipopolysaccharide increased the steady-state level of Il-1..beta.. mRNA. Glucocorticoids markedly decreased IL-1..beta.. mRNA levels by two mechanisms. Transcription of the IL-1 gene was inhibited, as shown by in vitro transcription assays with nuclei isolated from glucocorticoid-treated cells. Moreover, kinetic analyses and pulse-labeling of mRNAs showed that glucocorticoids selectively decrease the stability of IL-1..beta.. mRNA, without affecting the stability of ..beta..-actin and FOS mRNAs. Inhibition of the formation and effects IL-1 is a mechanism by which glucocorticoids can exert antiinflammatory and immunosuppressive effects.
- Research Organization:
- Institute of Biological Sciences, Palo Alto, CA (USA)
- OSTI ID:
- 6652080
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:4; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CULTURES
CHEMISTRY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ESTERS
GENES
GLUCOCORTICOIDS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSION
INHIBITION
ISOTOPES
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RNA
STEROIDS
TRANSCRIPTION
TRITIUM COMPOUNDS
URACILS
URIDINE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CULTURES
CHEMISTRY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ESTERS
GENES
GLUCOCORTICOIDS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSION
INHIBITION
ISOTOPES
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RNA
STEROIDS
TRANSCRIPTION
TRITIUM COMPOUNDS
URACILS
URIDINE
VERTEBRATES