Regulation of interleukin 3 mRNA expression in mast cells occurs at the posttranscriptional level and is mediated by calcium ions

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.2.777· OSTI ID:6719501

Wodnar-Filipowicz, A; Moroni, C ^[1]

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1. Univ. of Basel (Switzerland)

Interleukin 3 (IL-3) is transiently produced by murine bone marrow-derived mast cells in response to antigen stimulation of the high-affinity immunoglobulin E receptors. The authors have studied the postreceptor signaling pathways involved in regulating expression of the IL-3 gene in the murine mass cell PB-3c. Large amounts of IL-3 mRNA accumulated after exposure of cells to calcium ionophore A23187, a reagent that increases intracellular Ca{sup 2+}. Phorbol 12-myristate 13-acetate, which stimulates protein kinase C, did not induce IL-3 mRNA accumulation, although it did potentiate the effect of A23187. Nuclear run-on analysis showed that the IL-3 gene is constitutively transcribed in unstimulated cells and that treatment with A23187 and/or phorbol ester has no influence on its transcription rate. The effect of A23187 was found to be due to stabilization of the IL-3 mRNA. In cells maintained in the presence of A23187 the IL-3 mRNA was stable during 3 hr of incubation with actinomycin D, whereas removal of A23187 under the same conditions resulted in rapid degradation of the mRNA. These results indicate that control of expression of the IL-3 gene in mast cells is primarily at the posttranscriptional level and that the Ca{sup 2+}-dependent signal-transduction pathway plays an important role in this process. Synthesis of granulocyte/macrophage colony-stimulating factor mRNA in response to A23187 and phorbol ester was found to be subject to both transcriptional and posttranscriptional regulation.

• OSTI ID: 6719501
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:2; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BONE MARROW CELLS
CALCIUM COMPOUNDS
CARCINOGENS
CATIONS
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DRUGS
ELECTROPHORESIS
ESTERS
GENE REGULATION
GLOBULINS
GROWTH FACTORS
HYBRIDIZATION
IMMUNOGLOBULINS
IONS
ISOTOPES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAST CELLS
MEMBRANE PROTEINS
MESSENGER-RNA
MICE
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
RODENTS
SOMATIC CELLS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES