T cell clone exposed to high levels of interleukin-2 (IL-2) synthesizes reduced amounts of lymphokine mRNA in response to antigenic stimulation
The Mls-reactive, murine helper T cell clone, designated L2, both proliferates and secretes lymphokines including IL-2 and interleukin-3 (IL-3) when stimulated with alloantigen or with a combination of phorbol myristate acetate (PMA) and the divalent cation ionophore A23187. L2 cells were exposed to a low concentration (1 Unit(U)/ml) or to a high concentration (50-100 U/ml) of recombinant human IL-2 and cultured for 48-72 hours prior to restimulation with antigen or with PMA + A23187. Compared to L2 cells exposed to a low concentration of rIL-2, cells exposed to a high concentration of rIL-2 were profoundly less responsive to antigen as indicated a 6-16 fold reduction in antigen-stimulated lymphokine secretion. Consistent with the reduction in antigen-stimulated lymphokine secretion, levels of antigen-stimulated mRNA for both IL-2 and IL-3 were diminished 8-16 fold in L2 cells previously exposed to a high concentration of rIL-2. In contrast, L2 cells exposed to a high or low concentration of rIL-2 produced similar amounts of IL-2 and IL-3 when stimulated with PMA + A23187. These data suggest that exposure of L2 cells to a high concentration of IL-2 may inhibit the intracellular pathway which links antigen recognition to the production of lymphokine mRNA.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 5385286
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
CELL PROLIFERATION
LYMPHOKINES
SECRETION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ANTIGENS
BIOLOGICAL PATHWAYS
CATIONS
CLONE CELLS
MESSENGER-RNA
VALENCE
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CULTURES
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
ESTERS
GROWTH FACTORS
IONS
LEUKOCYTES
MATERIALS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RNA
SOMATIC CELLS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)