Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

T cell clone exposed to high levels of interleukin-2 (IL-2) synthesizes reduced amounts of lymphokine mRNA in response to antigenic stimulation

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5385286
; ;
The Mls-reactive, murine helper T cell clone, designated L2, both proliferates and secretes lymphokines including IL-2 and interleukin-3 (IL-3) when stimulated with alloantigen or with a combination of phorbol myristate acetate (PMA) and the divalent cation ionophore A23187. L2 cells were exposed to a low concentration (1 Unit(U)/ml) or to a high concentration (50-100 U/ml) of recombinant human IL-2 and cultured for 48-72 hours prior to restimulation with antigen or with PMA + A23187. Compared to L2 cells exposed to a low concentration of rIL-2, cells exposed to a high concentration of rIL-2 were profoundly less responsive to antigen as indicated a 6-16 fold reduction in antigen-stimulated lymphokine secretion. Consistent with the reduction in antigen-stimulated lymphokine secretion, levels of antigen-stimulated mRNA for both IL-2 and IL-3 were diminished 8-16 fold in L2 cells previously exposed to a high concentration of rIL-2. In contrast, L2 cells exposed to a high or low concentration of rIL-2 produced similar amounts of IL-2 and IL-3 when stimulated with PMA + A23187. These data suggest that exposure of L2 cells to a high concentration of IL-2 may inhibit the intracellular pathway which links antigen recognition to the production of lymphokine mRNA.
Research Organization:
Univ. of Chicago, IL
OSTI ID:
5385286
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

Similar Records

PMA and interleukin-1 do not necessarily perform the same function in the induction of IL-2 secretion and interleukin-2 receptor expression
Journal Article · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6829000
Effect of A-63162 on lymphocyte proliferation, IL-2 production, mRNA for Il-2 and LTB sub 4 production from human mononuclear cells
Conference · Thu Mar 14 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States) · OSTI ID:5242688
Some but not all T cell clones proliferate in response to PMA alone
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5457730

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CATIONS
CELL CULTURES
CELL PROLIFERATION
CHARGED PARTICLES
CLONE CELLS
CONNECTIVE TISSUE CELLS
ESTERS
GROWTH FACTORS
IONS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MESSENGER-RNA
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
RNA
SECRETION
SOMATIC CELLS
VALENCE