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PMA and interleukin-1 do not necessarily perform the same function in the induction of IL-2 secretion and interleukin-2 receptor expression

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6829000
;

The murine T-lymphoma cell line EL-4 was used to investigate the relative roles of interleukin-1 (IL-1), calcium, and activators of protein kinase C (PKC) in the induction of interleukin-2 (IL-2) secretion. EL-4 cells produced maximal levels of IL-2 in response to stimulation with 20 nM (but not 1 nM) phorbol myristate acetate (PMA). IL-1 and 1 nM PMA each synergized with calcium ionophore A23187, producing significant but submaximal levels of IL-2. Furthermore, an inhibitor of PKC inhibited IL-2 secretion stimulated by IL-1 and A23187. These results suggest that IL-1 and PMA both activate PKC in a similar manner. However, additional experiments indicated that IL-1 and PMA may have distinct mechanisms of promoting IL-2 production. IL-1 increased IL-2 production (and IL-2 receptor expression) induced by 1 nM PMA, 50 ..mu..M diacylglycerol, or combinations of either of these with A23187 to maximal levels. The synergy exhibited by IL-1 and low doses of PMA indicates that IL-1 and PMA may act at separate sites in the biochemical pathway, but high doses of PMA (20 nM) may replace the function of IL-1 as well.

Research Organization:
Smith Kline and French Labs., Philadelphia, PA
OSTI ID:
6829000
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ENZYME INHIBITORS
ESTERS
FUNCTIONS
GROWTH FACTORS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
RECEPTORS
RODENTS
SECRETION
SOMATIC CELLS
SYNERGISM
VERTEBRATES