Some but not all T cell clones proliferate in response to PMA alone

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5457730

Kim, D K; Moldwin, R L; Fitch, F W

The responses of murine T cell clones to PMA and A23187, were examined. Two cloned helper T lymphocytes (HTL) (L2 and J6.19) and two cloned cytolytic T lymphocytes (CTL) (L3 and B18) were cultured with IL-2 alone or with PMA and A23187, alone or in combination. The HTL proliferated in response to interleukin 2 (IL-2) and to the combination of PMA and A23187 but not to either agent alone. In contrast, the CTL proliferated in response to PMA alone, as well as to IL-2 or to the combination of PMA and A23187; like the HTL, the CTL did not proliferate in response to A23187 alone. The HTL released lymphokines IL-2 and macrophage-activating factor (MAF) in response to PMA + A23187 but not to either agent alone. Although the CTL proliferated in response to PMA alone, they did not release lymphokine MAF in response to PMA alone; like the HTL, the CTL did release MAF in response to the combination of PAM and A23187. PC61.5, an antibody directed against the murine-IL-2-receptor, completely inhibited HTL proliferation in response to IL-2 or to the combination of PMA and A23187. Although PC61.5 inhibited CTL proliferation induced by IL-2, it did not inhibit CTL proliferation induced by PMA alone. These results suggest that the proliferation of some HTL in response to PMA + A23187 is probably mediated by IL-2 via an autocrine mechanism. Some CTL can proliferate, apparently independently of IL-2, in response to PMA stimulation alone, and in the absence of lymphokine release.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5457730
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

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