Effect of A-63162 on lymphocyte proliferation, IL-2 production, mRNA for Il-2 and LTB sub 4 production from human mononuclear cells
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5242688
- Kansas State Univ., Manhattan (United States)
- Argonne National Labs., IL (United States)
Lipoxygenase metabolites of arachidonic acid have diversified effects on human lymphocytes. In the present investigation, the authors measured the effects of A-63162, a specific 5-lipoxygenase inhibitor on lymphocyte proliferation, IL-2 production, mRNA for IL-2 and LTB{sub 4} synthesis from peripheral blood human mononuclear cells. A-63162 inhibited the {sup 3}H-thymidine incorporation from PHA or PMA plus A23187 stimulated cultures. The synthesis of IL-2 was also inhibited from PHA plus PMA or PMA plus A23187 stimulated cultures. Further, they found A-63162 also inhibited the accumulation of IL-2 mRNA. And, A-63162 at that above concentration also inhibited the LTB{sub 4} synthesis from A23187 stimulated cultures. The results suggest that endogenous LTB{sub 4} may play an important role in regulating IL-2 production at the message level.
- OSTI ID:
- 5242688
- Report Number(s):
- CONF-9104107--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ENZYME INHIBITORS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RNA
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ENZYME INHIBITORS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MONOCYTES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RNA
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES