Radiation causes increased production and decreased utilization of IL-2 in human mononuclear cells

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Cytokine; (United States)

DOI:https://doi.org/10.1016/1043-4666(90)90041-Q· OSTI ID:5075185

Venkataraman, M; Westerman, M P ^[1]

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1. Department of Medicine, Mount Sinai Hospital Medical Center, Chicago, IL (United States)

The effects of radiation on the kinetics of Interleukin-2 (IL-2) production and utilization by mononuclear cells (MNCs) were studied. Mononuclear cells from normal, healthy individuals were subjected to various doses of radiation ranging from 0 to 2,000 rad and cultured in the presence of PHA. Supernatants from these cultures were harvested at various periods and their IL-2 contents determined by both the standard bioassay and ELISA. A radiation dose of 800 rad and higher had a marked effect on both IL-2 production and consumption. Although the supernatants from both the irradiated and non-irradiated MNCs contained maximal concentrations of IL-2 between 8 and 24 h of culture, the former had three times as much IL-2 as the latter. An increase in IL-2-mRNA levels was also noticed in irradiated, PHA-stimulated cells. Moreover, the supernatants from irradiated MNCs collected as late as 72 h after the initiation of culture contained more than 30% of the total IL-2 produced compared to less than 8% in supernatants from non-irradiated cells. Supernatants from non-irradiated cells incubated further with irradiated cells contained relatively higher quantities of IL-2 than those incubated continuously with non-irradiated cells. Supernatants from co-cultures of irradiated and non-irradiated MNCs contained less than expected amounts of IL-2 in two of the three subjects. Despite a difference in both the production and consumption of IL-2 between the irradiated and non-irradiated cells, there was no difference in their ability to generate IL-2 receptors. The results indicate that inactivation of radiosensitive suppressor T cells is associated with superinduction of IL-2 mRNA, increased production and decreased consumption of IL-2 by MNCs, thereby resulting in increased accumulation of IL-2.

• OSTI ID: 5075185
• Journal Information: Cytokine; (United States), Journal Name: Cytokine; (United States) Vol. 2:1; ISSN 1043-4666; ISSN XZ064
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ENZYME IMMUNOASSAY
GROWTH FACTORS
HEMAGGLUTININS
IMMUNOASSAY
IN VITRO
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
MITOGENS
MONOCYTES
MUCOPROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
REACTION KINETICS
RECEPTORS
RNA
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES