Exogenous IL-2 and copper (Cu) restore in vitro mitogenic reactivity of splenic mononuclear cells from copper deficient rats

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5214190

Bala, S; Deshpande, S; Failla, M ^[1]

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1. Dept. of Agriculture, Beltsville, MD (United States)

Male Lewis rats were nursed by dams fed a semipurified diet containing either adequate or deficient copper (Cu) after parturition and weaned to the identical diet. At eight weeks of age, splenic mononuclear cells (MNC) were isolated and blastogenic response to PHA was determined in the presence and absence of partially purified rat IL-2. PHA-induced blastogenesis by splenic MNC from Cu deficient rats was 35% that of MNC from Cu adequate rats. Addition of IL-2 to cultures increased blastogenesis of PHA-treated MNC from Cu deficient and control rats by 143% to 27%, respectively; mitogenic reactivity of IL-2 treated, PHA stimulated MNC from the two dietary groups were similar. Pre-incubation with monoclonal antibody against IL-2 receptor completely blocked PHA-induced blastogenesis in untreated and IL-2 treated cultures, demonstrating the IL-2 was the primary stimulator of T cell DNA synthesis. Preliminary studies have shown that the level of endogenous IL-2 in supernatants of mitogen stimulated MNC from Cu-deficient rats is lower than that in control cultures. In vitro supplementation of cultures with Cu also restored the mitogenic reactivity of MNC from Cu deficient rats. The effect of Cu supplementation on IL-2 secretion is being studied. The results suggest that dietary Cu deficiency reversibly impairs T cell function by decreasing the synthesis and/or secretion of IL-2 in response to activation.

• OSTI ID: 5214190
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
COPPER
DNA REPLICATION
ELEMENTS
GROWTH FACTORS
HEMAGGLUTININS
IN VITRO
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METABOLISM
METALS
MITOGENS
MONOCYTES
MUCOPROTEINS
NUCLEIC ACID REPLICATION
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN CELLS
TRANSITION ELEMENTS
VERTEBRATES