Normal gamma interferon (IFN-. gamma. ) and decreased interleukin-2 (IL-2) production by copper-deficient mice

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5144814

Lukasewycz, O A; Prohaska, J R ^[1]

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1. Univ. of Minnesota, Duluth (United States)

The production of both interleukin-2 (IL-2) and gamma interferon (IFN-{gamma}) was determined in lymphocyte preparations from spleens of copper-deficient ({minus}Cu) and copper adequate control (+Cu) mice. Swiss albino mice were fed a diet low in copper. The +Cu mice drank water with copper added, while {minus}Cu mice drank deionized water. Compared to +Cu controls, {minus}Cu mice had lower hematocrits, reduced levels of liver Cu, low plasma ceruloplasmin activity, and higher levels of liver iron. Production of IL-2 was assessed by the response of an IL-2-dependent cell line (CTLL) to serial dilutions of Con A-stimulated splenic lymphocyte culture supernatants. IFN-{gamma} levels were determined in these same supernatants by an enzyme-linked immunosorbant assay. Analysis indicated that IL-2 production by splenic lymphocytes from {minus}Cu mice was only 62% of the mean +Cu value. IFN-{gamma} levels of {minus}Cu and +Cu splenic lymphocytes, on the other hand, were equivalent. These data indicate differential effects of copper deficiency on two distinct lymphokines elaborated by the same murine T-help subpopulation, T{sub H}1.

• OSTI ID: 5144814
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CERULOPLASMIN
COMPLEXES
CONNECTIVE TISSUE CELLS
COPPER
COPPER COMPLEXES
ELEMENTS
ENZYME IMMUNOASSAY
GLOBULINS
GLOBULINS-ALPHA
GROWTH FACTORS
IMMUNOASSAY
INTERFERON
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
METABOLISM
METALLOPROTEINS
METALS
MICE
MITOGENS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES