Natural suppressor activity in graft-vs-host spleen and normal bone marrow is augmented by IL 2 and interferon-. gamma

Journal Article · Mon Dec 01 04:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6828830

Holda, J H; Maier, T; Claman, H N

Natural suppressor (NS) cells isolated from the spleens of mice 34 or more days after induction of chronic graft-vs-host disease showed potent suppressor activity that was dependent on the presence of lymphokines such as those found in lectin-free Con A supernatant (CAS). Both IL 2 and IFN-..gamma.. were found to enhance NS activity, with IFN-..gamma.. being the most active molecule. To generalize these findings, normal adult bone marrow (BM), a second environment where NS activity has been reported, was examined. Normal adult BM cells nonspecifically suppressed both B and T cell mitogen responses. BM suppression could also be enhanced by the addition of CAS to the cultures. Removal of plastic-adherent cells, Thy-1.2-positive cells, or B cells did not significantly diminish suppression or remove the ability to enhance suppression with CAS. When isolated on discontinuous Percoll gradients, BM suppressor cells banded in the less dense fractions, and the suppression in these bands was also enhanced by lymphokines. The suppressive activity of these cells was greatly enhanced by the addition of CAS, IFN-..gamma.., or IL 2. However, IFN-..gamma.. appears to be a more potent enhancing molecule, being active at levels between 1 and 10 U/ml.

• Research Organization: Univ. of Colorado School Medicine, Denver
• OSTI ID: 6828830
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 137:11; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
COBALT 60
COBALT ISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
FEMALES
GAMMA RADIATION
GRAFT-HOST REACTION
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSIVE DRUGS
INTERFERON
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LYMPHOKINES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHARMACOLOGY
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES