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Molecular and genetic characterization of the RAD3 gene of Saccharomyces cerevisiae

Thesis/Dissertation ·
OSTI ID:6901375

The RAD3 gene of Saccharomyces cerevisiae is involved in the excision repair of damaged DNA. Strains carrying a radS mutation are highly sensitive to certain types of DNA damage. A fragment of yeast DNA which complemented the UV-light sensitivity of a rad3 strain was isolated and shown by genetic evidence to be the RAD3 gene. Using this cloned gene, it was shown that RAD3 is essential for the viability of haploid cells under normal growth conditions. The RAD3 gene is not inducible by DNA damage. The sequence of the RAD3 gene predicted a protein of 90kd, with a highly negatively charged carboxy terminus, however, deletion analysis demonstrated that this region was not required for either function of RAD3. The sequence also showed some homology to nucleotide binding proteins and to DNA binding proteins.

Research Organization:
Stanford Univ., CA (USA)
OSTI ID:
6901375
Country of Publication:
United States
Language:
English

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Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
FUNGI
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
MICROORGANISMS
MOLECULAR STRUCTURE
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION
YEASTS