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RAD3 gene of Saccharomyces cerevisiae encodes a DNA-dependent ATPase

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;

The RAD3 gene of the yeast Saccharomyces cerevisiae is required for excision repair of damaged DNA and for cell viability. A protein of approx. = 89 kDa was purified to near homogeneity from yeast strains harboring multicopy plasmids that overproduce RAD3 protein; this protein corresponds closely to the expected size of the RAD3 protein and crossreacts with the antiserum raised against a truncated RAD3 protein produced in Escherichia coli. The purified RAD3 protein shows a single-stranded DNA-dependent ATPase activity that catalyzes hydrolysis of ATP to ADP and P/sub i/. The ATPase activity was coincident with the RAD3 protein during purification and is inhibited by anti-RAD3 antibodies, indicating that the RAD3 gene encodes this activity

Research Organization:
Univ. of Rochester, NY (USA)
DOE Contract Number:
FG02-85ER60281
OSTI ID:
5209839
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:17; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ATP
ATP-ASE
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DNA REPAIR
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
FUNGI
GENES
GENETICS
HYDROLASES
HYDROLYSIS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
PROTEINS
PURIFICATION
RADIOISOTOPES
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
TRITIUM COMPOUNDS
YEASTS