DNAter dot RNA helicase activity of RAD3 protein of Saccharomyces cerevisiae

Journal Article · Fri Nov 01 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.21.9712· OSTI ID:5821412

Bailly, V; Sung, P; Prakash, L; Prakash, S ^[1]

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1. Univ. of Rochester, NY (United States)

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair of UV-damaged DNA and is essential for cell viability. The RAD3 protein exhibits a remarkable degree of sequence homology to the human excision repair protein ERCC2. The RAD3 protein is a single-stranded DNA-dependent ATPase and a DNA helicase capable of denaturing long regions of duplex DNA. Here, the authors demonstrate that RAD3 also possesses a potent DNA{center dot}RNA helicase activity similar in efficiency to its DNA helicase activity. The rad3 Arg-48 mutant protein, which binds but does not hydrolyze ATP, lacks the DNA{center dot}RNA unwinding activity, indicating a dependence on ATP hydrolysis. RAD3 does not show any RNA-dependent NTPase activity and, as expected, does not unwind duplex RNA. This observation suggest that RAD3 translocates on DNA in unwinding DNA{center dot}RNA duplexes. That the rad3 Arg-48 mutation inactivates the DNA and DNA{center dot}RNA helicase activities and confers a substantial reduction in the incision of UV-damaged DNA suggests a role for these activities in incision. The authors discuss how RAD3 helicase activities could function in tracking of DNA in search of damage sites and effect enhanced excision repair of actively transcribed genes.

• OSTI ID: 5821412
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:21; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DAYS LIVING RADIOISOTOPES
DNA
DNA HELICASES
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EUMYCOTA
EXCISION REPAIR
FUNGI
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
YEASTS