Repair of UV damaged DNA, genes and proteins of yeast and human
Our objectives are to determine the molecular mechanism of the incision step of excision repair of ultraviolet (UV) light damaged DNA in eukaryotic organisms, using the yeast Saccharomyces cerevisiae as a model system, as well as studying the human homologs of yeast excision repair and postreplication repair proteins. In addition to its single-stranded DNA-dependent A TPase and DNA helicase activities, we have found that RAD3 protein also possesses DNA-RNA helicase activity, and that like RAD3, the Schizosaccharomyces pombe RAD3 homolog, rhp3{sup +}, is an essential gene. We have overexpressed the human RAD3 homolog, ERCC2, in yeast to facilitate its purification. The RAD10 protein was purified to homogeneity and shown to bind DNA. ERCC3y, the yeast homolog of the human ERCC-3/XP-B gene, has been sequenced and shown to be essential for viability. The Drosophila and human homologs of RAD6, required for postreplication repair and UV induced mutagenesis, were shown to complement the rad6 {Delta} mutation of yeast. Since defective DNA repair and enhanced neoplasia characterize several human genetic diseases, and repair proteins are highly conserved between yeast and man, a thorough understanding of the molecular mechanisms of DNA repir in yeast should provide a better understanding of the causes of carcinogenesis.
- Research Organization:
- Rochester Univ., NY (USA). School of Medicine and Dentistry
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG02-88ER60621
- OSTI ID:
- 5763197
- Report Number(s):
- DOE/ER/60621-4; ON: DE91012170
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
DNA REPAIR
DNA SEQUENCING
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EUMYCOTA
EXCISION REPAIR
FRACTIONATION
FUNGI
GENE MUTATIONS
GENE OPERONS
GENE RECOMBINATION
GENE RECOMBINATION PROTEINS
GENES
GENETICS
MICROORGANISMS
MUTATIONS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLANTS
PROGRESS REPORT
PROTEINS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
ULTRAVIOLET RADIATION
YEASTS