Nucleotide excision repair of DNA in the yeast S. cerevisiae

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5269300

Friedberg, E C

In the yeast S. cerevisiae the excision of pyrimidine dimers (produced by ultraviolet radiation) from DNA is apparently a complex biochemical process. Genetic studies indicate the involvement of at least 10 genes, 5 of which are absolutely required for the incision of DNA during excision repair. The authors have isolated these genes (RAD1, RAD2, RAD3, RAD4, RAD10) by molecular cloning. Sequencing has been completed on 4 of the genes and has provided information on the sizes of the proteins presumably encoded by them. In addition, regions of amino acid sequence homology with other proteins suggest evolutionary and functional relationships. To study aspects of the regulation of expression of these genes the authors have constructed RAD-lacZ fusions and have also quantitatively analyzed transcription of some of the RAD genes. These studies showed that the RAD2 gene undergoes enhanced transcription following exposure of cells to a variety of DNA damaging agents or agents which cause arrested DNA replication. The RAD1, RAD3, RAD4 and RAD10 genes are not induced under these conditions. Deletion analysis has shown that the RAD3 gene is essential for the viability of haploid yeast cells. Overexpression of the RAD3 and RAD2 genes in E. coli facilitated the raising of specific rabbit antisera to Rad3 and Rad2 proteins, and overexpression of the RAD3 gene in yeast has facilitated partial purification of Rad3 protein.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 5269300
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
FUNGI
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACID REPLICATION
NUCLEOTIDES
ORGANIC COMPOUNDS
PLANTS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
ULTRAVIOLET RADIATION
YEASTS