Regulation of inositol phosphate levels by prostaglandins in cultured endometrial cells

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041280116· OSTI ID:6898682

Orlicky, D J; Silio, M; Williams, C; Gordon, J; Gerschenson, L E

Stimulation of cultured rabbit endometrial cells by one of the rabbit endometrial cell culture proliferation factors, prostaglandin F/sub 2//sub ..cap alpha../ (PGF/sub 2//sub ..cap alpha../), resulted in a very rapid increase in the intracellular levels of (/sup 3/H)-inositol triphosphate (IP/sub 3/), (/sup 3/H)-inositol biphosphate (IP/sub 2/), and (/sup 3/H)-inositol monophosphate (IP/sub 1/) in cells prelabeled with (/sup 3/H)-inositol. These increases in inositol phosphate levels were detected in periods of stimulation as short as 30 seconds, reached a maximum by 1 1/2-2 min and declined to control levels by 6-10 min. The stimulation was dose-dependent with maximal increases observed near 10/sup -6/ M PGF/sub 2//sub ..cap alpha../. The cholinergic agent, carbachol, also led to time and dose-independent increases in IP/sub 3/. Lithium, cadmium, silver, copper, and zinc ions had no effect either on the breakdown of IP/sub 3/ or on the accumulation of IP/sub 1/. In contrast, vanadate at 10/sup -6/ or 10/sup -5/ M did lead to a decrease in the breakdown of IP/sub 1/ and a concomitant increase in IP/sub 1/, IP/sub 2/, and IP/sub 3/. PGF/sub 2//sub ..cap alpha../ was found previously to induce an increase in rabbit endometrial cell DNA synthesis which was inhibited by concomitant or prior addition of prostaglandin E/sub 1/ (PGE/sub 1/). PGE/sub 1/, in a dose-dependent manner, was found to inhibit the observed IP/sub 3/ increase by PGF/sub 2//sub ..cap alpha../ at 1 1/2 min of stimulation. PGF/sub 2//sub ..cap alpha../ treated and control cultures did not differ in cAMP 3 or cGMP levels, cellular /sup 45/Ca uptake, nor cellular /sup 22/Na uptake. The authors propose that P/sub 3/ may be one of the intracellular messenger(s) synthesized following the treatment of rabbit endometrial cell cultures with the proliferation agent PGF/sub 2//sub ..cap alpha../ and that it may play a crucial role with cAMP in growth regulation.

• Research Organization: Univ. of Colorado Health Sciences Center, Denver
• OSTI ID: 6898682
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 128:1; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKALINE EARTH ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARBOHYDRATES
CELL CULTURES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FEMALE GENITALS
INOSITOL
INOSITOLS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHYSIOLOGY
PROSTAGLANDINS
RABBITS
RADIOISOTOPES
SACCHARIDES
SODIUM 22
SODIUM ISOTOPES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
UPTAKE
UTERUS
VANADATES
VANADIUM COMPOUNDS
VERTEBRATES
YEARS LIVING RADIOISOTOPES