Involvement of prostaglandins F/sub 2. cap alpha. / and E/sub 1/ with rabbit endometrium

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5009030

Orlicky, D J

Several growth factors and hormones are thought to play a role in the growth control of endometrial cells. The authors have shown that prostaglandin F/sub 2..-->../ (PGF/sub 2..cap alpha../) is a growth factor for primary cultures of rabbit endometrium cultured in chemically-defined serum-free medium and that prostaglandin E/sub 1/ (PGE/sub 1/) antagonizes the PGF/sub 2..-->../ induction of growth. Both (/sup 3/H)PGF/sub 2..cap alpha../ and (/sup 3/H)PGE/sub 1/ bind in a time and temperature dependent, dissociable, saturable and specific manner. The binding of (/sup 3/H)PGF/sub 2..cap alpha../ and (/sup 3/H)PGE/sub 1/ can be both down and up regulated and is enzyme sensitive. PGE /sub 1/ stimulates intracellular cAMP synthesis and accumulation in a time and concentration dependent manner. PGF/sub 2..cap alpha../ probably exerts its effects through an amiloride-sensitive intermediate. Both PGF/sub 2..cap alpha../ and PGE/sub 1/ are constitutively synthesized by these primary cultures, and they have shown this synthesis to be both drug and hormone sensitive. They hypothesize that it is the ratio, rather than the absolute quantities, of PGF/sub 2..cap alpha../ and PGE/sub 1/ which is of more importance in the regulation of endometrial cell growth. Furthermore, they believe this regulation of endometrial growth plays a role in control of proliferation during the decidual response and that a derangement in the ratio of these prostaglandins may lead to either infertility or hyperplasia. The ability of these cultures to synthesize prostaglandins in a hormonally regulatable manner may be of importance in the study of dysmenorrhea and uterine cramping as caused by the myometrial contracting prostaglandin, PGF/sub 2..cap alpha../.

• Research Organization: Colorado Univ., Denver (USA). Health Sciences Center
• OSTI ID: 5009030
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMAL TISSUES
ANIMALS
BIOSYNTHESIS
BODY
CELL CULTURES
CELL PROLIFERATION
EPITHELIUM
FEMALE GENITALS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
RABBITS
SYNTHESIS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES