Comparison of specific binding sites for Escherichia coli RNA polymerase with naturally occurring hairpin regions in single-stranded DNA of coliphage M13. [Aspergillus oryzae]

Journal Article · Fri Aug 25 04:00:00 EDT 1978 · J. Biol. Chem.; (United States)

OSTI ID:6894005

Niyogi, S K; Mitra, S

Escherichia coli RNA polymerase binds specifically to the single-stranded circular DNA of coliphage M13 in the presence of a saturating concentration of the bacterial DNA binding protein presumably as an essential step in the synthesis of the RNA primer required for synthesizing the complementary DNA strand in parental replicative-form DNA. The RNA polymerase-protected DNA regions were isolated after extensive digestion with pancreatic DNase, S1 endonuclease of Aspergillus oryzae, and exonuclease I of E. coli. The physicochemical properties of the RNA polymerase-protected segments (called PI and PII) were compared with those of the naturally occurring hairpin regions.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 6894005
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 253:16; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ASPERGILLUS
BACTERIA
BACTERIOPHAGES
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
DNA
DNA-ASE
ENERGY
ENZYMES
ESCHERICHIA COLI
ESTERASES
FUNGI
HYDROLASES
KINETICS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHATASES
PHOSPHOTRANSFERASES
PLANTS
POLYMERASES
REACTION KINETICS
RNA
TRANSFERASES
VIRUSES