Comparison of specific binding sites for Escherichia coli RNA polymerase with naturally occurring hairpin regions in single-stranded DNA of coliphage M13. [Aspergillus oryzae]
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6894005
Escherichia coli RNA polymerase binds specifically to the single-stranded circular DNA of coliphage M13 in the presence of a saturating concentration of the bacterial DNA binding protein presumably as an essential step in the synthesis of the RNA primer required for synthesizing the complementary DNA strand in parental replicative-form DNA. The RNA polymerase-protected DNA regions were isolated after extensive digestion with pancreatic DNase, S1 endonuclease of Aspergillus oryzae, and exonuclease I of E. coli. The physicochemical properties of the RNA polymerase-protected segments (called PI and PII) were compared with those of the naturally occurring hairpin regions.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6894005
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 253:16
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13
Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13
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Journal Article
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Wed Dec 21 00:00:00 EST 1977
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6894005
Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13
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Sat Jan 01 00:00:00 EST 1977
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6894005
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Journal Article
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Sun Jan 01 00:00:00 EST 1978
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6894005
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
DNA-ASE
NUCLEASES
POLYMERASES
RNA
ASPERGILLUS
BACTERIOPHAGES
ESCHERICHIA COLI
BACTERIA
ENERGY
ENZYMES
ESTERASES
FUNGI
HYDROLASES
KINETICS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHATASES
PHOSPHOTRANSFERASES
PLANTS
REACTION KINETICS
TRANSFERASES
VIRUSES
550200* - Biochemistry
DNA
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
DNA-ASE
NUCLEASES
POLYMERASES
RNA
ASPERGILLUS
BACTERIOPHAGES
ESCHERICHIA COLI
BACTERIA
ENERGY
ENZYMES
ESTERASES
FUNGI
HYDROLASES
KINETICS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHATASES
PHOSPHOTRANSFERASES
PLANTS
REACTION KINETICS
TRANSFERASES
VIRUSES
550200* - Biochemistry