Structure of nascent replicative form DNA of coliphage M13
Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' ..-->.. 3' exonuclease associated with DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase and T4 phage DNA ligase. These RFI molecules are labile to alkali and RNase H, unlike the RFI produced either in vivo or from RFII with E. coli DNA polymerase I and E. coli DNA ligase. The ribonucleotides are located at one site and predominantly in one strand of the nascent RF DNA. Furthermore, these molecules contain multiple small gaps, randomly located, and one large gap in the intracistronic region.
- Research Organization:
- Univ. of Tennessee-Oak Ridge National Lab., TN
- OSTI ID:
- 6645800
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 75:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
MOLECULAR STRUCTURE
BACTERIOPHAGES
DNA REPLICATION
ESCHERICHIA COLI
GENES
GENETICS
IN VITRO
LIGASES
MOLECULAR BIOLOGY
MUTANTS
NUCLEASES
NUCLEOTIDES
POLYMERASES
BACTERIA
BIOLOGY
ENZYMES
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
TRANSFERASES
VIRUSES
550400* - Genetics
550200 - Biochemistry