Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13

Journal Article · Wed Dec 21 04:00:00 EST 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)91109-3· OSTI ID:5166984

Niyogi, S K; Mitra, S

With precise conditions of digestion with single-strand-specific nucleases, namely, endonuclease S1 of Aspergillus oryzae and exonuclease I of Escherichia coli, nuclease-resistant DNA cores can be obtained reproducibly from single-stranded M13 DNA. The DNA cores are composed almost exclusively of two sizes (60 and 44 nucleotides long). These have high (G + C)-contents relative to that of intact M13 DNA, and arise from restricted regions of the M13 genome. The resistance of these fragments to single-strand-specific nucleases and their nondenaturability strongly suggest the presence of double-stranded segments in these core pieces. That the core pieces are only partially double-stranded is shown by their lack of complete base complementarity and their pattern of elution from hydroxyapatite.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5166984
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 79:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ASPERGILLUS
BACTERIA
BACTERIOPHAGES
BIOCHEMISTRY
BIOLOGY
BIOMASS
CHEMISTRY
DNA
ENERGY SOURCES
ENZYMES
ESCHERICHIA COLI
FUNGI
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
PLANTS
RENEWABLE ENERGY SOURCES
TRANSFERASES
VIRUSES