Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)91109-3· OSTI ID:6621373

Niyogi, S K; Mitra, S

With precise conditions of digestion with single-strand-specific nucleases, namely, endonuclease S1 of Aspergillus oryzae and exonuclease I of Escherichia coli, nuclease-resistant DNA cores can be obtained reproducibly from single-stranded M13 DNA. The DNA cores are composed almost exclusively of two sizes (60 and 44 nucleotides long). These have high (G + C)-contents relative to that of intact M13 DNA, and arise from restricted regions of the M13 genome. The resistance of these fragments to single-strand-specific nucleases and their nondenaturability strongly suggest the presence of double-stranded segments in these core pieces. That the core pieces are only partially double-stranded is shown by their lack of complete base complementarity and their pattern of elution from hydroxyapatite.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 6621373
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 79:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMISTRY
CHEMISTRY
DNA
DNA-ASE
ENZYMES
ESCHERICHIA COLI
ESTERASES
HYDROLASES
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHATASES
RIBOSIDES
STRUCTURAL CHEMICAL ANALYSIS