Effects of N-dodecane pretreatment on the metabolism and distribution of benzo(a)pyrene in the isolated perfused rabbit lung

Journal Article · Mon Nov 17 04:00:00 EST 1980 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(80)90427-0· OSTI ID:6879946

Warshawsky, D ^[1]; Bingham, E; Niemeier, R W

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1. Univ. of Cincinnati Medical College, OH

B(a)P is an ubiquitous potent carcinogen that has been associated with the increased incidence of human bronchiogenic carcinoma in occupational and urban populations. An isolated perfused rabbit lung preparation (IPL) was used to study the influence of pretreatment with a cocarcinogen, n-dodecane, on the metabolism of benzo(a)pyrine (B(a)P). /sup 14/C-B(a)P was administered intratracheally to the IPL following biweekly inhalation exposure of the rabbits to n-dodecane. Results from n-dodecane pretreatment group were compared to those from the no-pretreatment group and an intratracheally administered B(a)P pretreatment group (B(a)P IT). Metabolites were isolated from serial blood samples up to 3 hours after administration of the /sup 14/C-B(a)P to the IPL. Patterns of B(a)P metabolites were determined using thin layer and high performance liquid chromatography, and liquid scintillation counting. The rates of appearance of B(a)P metabolites in the blood and the B(a)P metabolic pattern for n-dodecane groups were compared to B(a)P IT and no-pretreatment groups. The rates of appearance of metabolites in the blood (ng/hr/g of lung) were similar for B(a)P IT and n-dodecane pretreatment groups, but were five and four times greater, respectively, when compared to rates of appearance of metabolites for the no-pretreatment group. Significance of the findings is that a known cocarcinogen, n-dodecane, appears to be as good an enzyme inducer as B(a)P in stimulating metabolism of B(a)P in lung. Therefore, man's risk in developing cancer may not be solely a result of exposure to a carcinogen, but may be dependent upon other constituents in his environment.

• OSTI ID: 6879946
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 27:20; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMALS
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBON 14
CARBON ISOTOPES
CARCINOGENS
CHROMATOGRAPHY
CONDENSED AROMATICS
COUNTING TECHNIQUES
DODECANE
EVEN-EVEN NUCLEI
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LIQUID SCINTILLATORS
LUNGS
MAMMALS
MATERIALS
METABOLISM
METABOLITES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHOSPHORS
PUBLIC HEALTH
RABBITS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SCINTILLATION COUNTING
SEPARATION PROCESSES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES