Effects of a cocarcinogen, ferric oxide, on the metabolism of benzo(a)pyrene in the isolated perfused lung

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398409530573· OSTI ID:5885183

Warshawsky, D; Bingham, E

An isolated perfused New Zealand rabbit lung preparation was used to investigate the effects of a cocarcinogen, ferric oxide (Fe/sub 2/O/sub 3/), on the metabolism of benzo(a)pyrene (BaP), a ubiquitous potent carcinogen that has been associated with the increased incidence of human bronchiogenic carcinoma in occupational and urban settings. (/sup 14/C)-BaP was administered intratracheally to an isolated perfused lung (IPL) preparation with and without Fe/sub 2/O/sub 3/ after intraperitoneal pretreatment of the whole animal with BaP or intratracheal pretreatment of the whole animal with Fe/sub 2/O/sub 3/ and/or BaP. BaP and its metabolites were isolated from serial blood samples up to 180 min after administration of (/sup 14/C)BaP to the IPL. BaP and its metabolites were also isolated from lung tissue, washout fluid, macrophage, and trachea bronchi at the end of the perfusion at 180 min. Patterns of BaP metabolites were determined by chromatographic techniques and liquid scintillation counting. Fe/sub 2/O/sub 3/ pretreatment to the whole animal or administration of Fe/sub 2/O/sub 3/ to the IPL altered BaP metabolism by the perfused lung. Fe/sub 2/O/sub 3/ pretreatment to the whole animal resulted in an increase in the total rate of appearance of metabolites of BaP in the blood, while Fe/sub 2/O/sub 3/ administration to the IPL resulted in a decrease in the total rate of appearance of BaP metabolites in the blood and inhibited the effect of pretreatment. These data suggest that pulmonary exposure to a known cocarcinogen, Fe/sub 2/O/sub 3/, in the presence of BaP results in increased production of dihydrodiols of BaP, which may be further metabolized to the ultimate carcinogenic form(s) of BaP. Therefore, Fe/sub 2/O/sub 3/ can enhance the metabolic activation of BaP by the lung, as well as act as a carrier for penetration and retention of BaP in the lung. 49 references, 6 tables.

• Research Organization: Univ. of Cincinnati Medical Center, OH
• OSTI ID: 5885183
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 14:2-3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
BRONCHI
CARBON 14 COMPOUNDS
CARCINOGENESIS
CARCINOMAS
CHALCOGENIDES
CHROMATOGRAPHY
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DATA
DISEASES
EXPERIMENTAL DATA
HYDROCARBONS
INFORMATION
INTRATRACHEAL ADMINISTRATION
IRON COMPOUNDS
IRON OXIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MACROPHAGES
MAMMALS
METABOLIC ACTIVATION
METABOLITES
NEOPLASMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOGENESIS
PERFUSED ORGANS
PHAGOCYTES
RABBITS
RESPIRATORY SYSTEM
RETENTION
SCINTILLATION COUNTING
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES