Influence of sulfur dioxide on metabolism and distribution of benzo(a)pyrene in isolated perfused rabbit lung

Journal Article · Mon Jun 01 04:00:00 EDT 1981 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398109530041· OSTI ID:6251365

Warshawsky, D ^[1]; Niemeier, R; Bingham, E

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1. Univ. of Cincinnati, OH

An isolated perfused lung (IPL) preparation was used to investigate the effects of SO/sub 2/ (1 to 2 ppM) on the metabolism of benzo(a)pyrene (BaP), a ubiquitous potent carcinogen that has been associated with the increased incidence of human brochiogenic carcinoma in occupational and urban populations. (/sup 14/C)BaP, with and without crude air particulate (CAP), was administered intracheally to the IPL in conjunction with SO/sub 2/ or after pretreatment of the whole animal with SO/sub 2/. Metabolites were isolated from serial blood samples up to 3 h after the administration of (/sup 14/C)BaP to the IPL. Metabolites were also isolated from lung tissue, washout fluid, macrophage, and trachea and bronchi at the end of the perfusion at 180 min. Patterns of BaP metabolites were determined by thin-layer and high-performance liquid chromatography and liquid scintillation counting. SO/sub 2/ given in conjunction with BaP on the IPL or given to the whole animal followed by BaP on the IPL, in comparison with BaP only on the IPL, resulted in a twofold increase in the total rate of appearance of metabolites of BaP in the blood with changes in the metabolic pattern. SO/sub 2/ given in conjunction with BaP and CAP on the IPL, in comparison with BaP plus SO/sub 2/ on the IPL, resulted in a threefold decrease in the total rate of appearance of metabolites of BaP in the blood with changes in the metabolic pattern.

• OSTI ID: 6251365
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 7:6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTROSCOPY
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CHALCOGENIDES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DATA
DYNAMIC FUNCTION STUDIES
EXPERIMENTAL DATA
HYDROCARBONS
INFORMATION
INTRATRACHEAL ADMINISTRATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MACROPHAGES
MAMMALS
MATERIALS
METABOLISM
METABOLITES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PARTICLES
PARTICULATES
PERFUSED ORGANS
PHAGOCYTES
QUALITATIVE CHEMICAL ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RABBITS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
SEPARATION PROCESSES
SOMATIC CELLS
SPECTROSCOPY
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR OXIDES
TRACER TECHNIQUES
VERTEBRATES