Interactions of heavy metals with the pulmonary metabolism of (3H)benzo(a)pyrene

Technical Report · Sun Jan 01 04:00:00 EST 1984

OSTI ID:6935050

Williams, S J; Karis, M A; Menzel, D B

To test the hypothesis that the inhalation of heavy-metal aerosols from polluted air or cigarette smoke could alter the metabolism of polycyclic aromatic hydrocarbons, isolated, ventilated, and perfused rat lungs were used to study the effects of NiCl/sub 2/, CdCl/sub 2/, and CoCl/sub 2/ on the pulmonary metabolism of (3)H benzo(a)pyrene (3)H BAP. Five minutes prior to removing the lungs from a rat, 100 microliter of isotonic sucrose containing 1 micromole of NiCl/sub 2/, CdCl/sub 2/, or CoCl/sub 2/ was instilled intratracheally. The lungs were placed in the perfusion system and washed free of blood. After 10 minutes, 5 nmole of (3)H BAP dissolved in 100 microliter of dimethylsulfoxide was introduced in the arterial circulation. After 45 minutes the lungs were homogenized and the homogenates and perfusates were extracted with ethyl acetate: (2:1, v/v) for analysis by HPLC. Binding of (3)H BAP metabolites to lung tissue was also determined. The metabolism of (3)H BAP was found to be inducible by pretreatment of rats with beta-naphthoflavone (80 mg/kg) and to be inhibited by the inclusion of metyrapone (1.0 mM) or indomethacin (0.1 mM) in the perfusate. The extent of covalent binding to lung macromolecules was proportional to the extent of metabolism of the (3)H BAP. Since heavy metals and polycyclic aromatic hydrocarbon coexist in the same atmospheric aerosols, heavy metal exposure from polluted urban or occupational air or cigarette smoking may have profound effects upon the bioactivity of a common organic carcinogen.

• Research Organization: Duke Univ., Durham, NC (USA). Medical Center
• OSTI ID: 6935050
• Report Number(s): PB-87-145546/XAB
• Country of Publication: United States
• Language: English

Related Subjects

500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AROMATICS
BENZOPYRENE
CARCINOGENS
CHLORIDES
CHLORINE COMPOUNDS
COLLOIDS
CONDENSED AROMATICS
DISPERSIONS
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
HYDROCARBONS
INHALATION
INTAKE
LABORATORY ANIMALS
METABOLISM
METALS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
SOLS