Interactions of heavy metals with the pulmonary metabolism of (/sup 3/H)benzo(a)pyrene

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Environ. Res.; (United States)

OSTI ID:5995201

Williams, S J; Karis, M A; Menzel, D B

To test the hypothesis that the inhalation of heavy metal aerosols from polluted air or cigarette smoke could alter the metabolism of polycyclic aromatic hydrocarbons, isolated, ventilated, and perfused rat lungs were used to study the effects of NiCl/sub 2/, CdCl/sub 2/, and CoCl/sub 2/ on the pulmonary metabolism of (/sup 3/H)benzo(a)pyrene ((/sup 3/H)BAP). Five minutes prior to removing the lungs from a rat, 100 ..mu..l of isotonic sucrose containing 1 ..mu..mole of NiCl/sub 2/, CdCl/sub 2/, or CoCl/sub 2/ was instilled intratracheally. The lungs were placed in the perfusion system and washed free of blood. After 10 min, 5 nmole of (/sup 3/H)BAP dissolved in 100 ..mu..l of dimethylsulfoxide was introduced in the arterial circulation. After 45 min, the lungs were homogenized and the homogenates and perfusates were extracted with ethyl acetate:acetone (2:1, v/v) or analysis by HPLC. Binding of (/sup 3/H)BAP metabolites to lung tissue was also determined. The metabolism of (/sup 3/H)BAP was found to be inducible by pretreatment of rats with ..beta..-naphthoflavone (80 mg/kg) and to be inhibited by the inclusion of metyrapone (1.0 mM) or indomethacin (0.1 mM) in the perfusate. Instillation of NiCl/sub 2/ resulted in a slight inhibition of metabolism. In the presence of CdCl/sub 2/, 20% or more of the parent compound was metabolized compared to sucrose-treated controls, but the fraction of organic solvent extractable metabolites increased to 213% of controls. CoCl/sub 2/ was without effect on (/sup 3/H)BAP metabolism. In all cases, the extent of covalent binding to lung macromolecules was proportional to the extent of metabolism of the (/sup 3/H)BAP.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 5995201
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 34:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AIR POLLUTION
ANIMALS
AROMATICS
BENZOPYRENE
BODY
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHEMICAL BONDS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
COBALT CHLORIDES
COBALT COMPOUNDS
COLLOIDS
CONDENSED AROMATICS
DATA
DISPERSIONS
EXPERIMENTAL DATA
HALIDES
HALOGEN COMPOUNDS
HYDROCARBONS
INFORMATION
INHALATION
INTAKE
INTRATRACHEAL ADMINISTRATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
METABOLITES
NICKEL CHLORIDES
NICKEL COMPOUNDS
NUMERICAL DATA
OCCUPATIONAL EXPOSURE
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RESIDUES
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
RODENTS
SMOKES
SOLS
TOBACCO SMOKES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
URBAN AREAS
VARIATIONS
VERTEBRATES