In vitro synthesis of DNA, protein, and lipids by the de-endothelialized rabbit aorta

Journal Article · · Arteriosclerosis (Dallas); (United States)

DOI:https://doi.org/10.1161/01.ATV.1.6.418· OSTI ID:6871557

Rosenfeld, R S; Drouet, L; Cintron, J; Paul, I; Won, J; Spaet, T H

The uptake of (/sup 3/H) leucine, (/sup 3/H) thymidine, (/sup 14/C) acetate, and (/sup 14/C) mevalonic acid by aortic intima media from normal rabbits and from rabbits subjected to a single balloon de-endothelialization as measured 6 days, 2 months, and 4 months after treatment to determine how long the injury-induced stimulation of incorporation of these precursors into tissue components persisted beyond 6 days, the time of maximum proliferative response of the smooth muscle cells. We found that (/sup 3/H) thymidine incorporation (indicative of DNA synthesis and the potential for cell proliferation) was about three times greater in the de-endothelialized tissue than in the control tissue 6 days after vessel injury, but that by 2 months it was normal. Labeled leucine incorporation into the de-endothelialized tissue (a measure of protein synthesis) was eight times higher than normal at the time of maximum proliferative response to injury (6 days), and showed no decrease under identical incubation conditions in ballooned tissue obtained 2 months after de-endothelialization; it continued high at 4 months. Both (/sup 14/C) acetate and (/sup 14/C) mevalonate uptake into lipids by the aortic tissue were enhanced by the injury; this increased incorporation was still evident at 4 months. Thus, de-endothelialization of the aorta triggers a vessel wall response characterized by augmented protein synthesis and lipogenesis, which persists at least 4 months after injury and at least 2 months after DNA synthesis has normalized.

• Research Organization: Institute for Steroid Research and Division of Hematology, Montefiore Hospital and Medical Center, Albert Einstein College of Medicine, Bronx, NY
• OSTI ID: 6871557
• Journal Information: Arteriosclerosis (Dallas); (United States), Journal Name: Arteriosclerosis (Dallas); (United States) Vol. 1:6; ISSN ARTRD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETATES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
AZINES
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DNA
ENDOTHELIUM
HETEROCYCLIC COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGY
PHYSIOLOGY
PYRIMIDINES
RABBITS
RIBOSIDES
SYNTHESIS
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES