Incorporation of fucose and leucine into PNS myelin proteins in nerves undergoing early Wallerian degeneration

Journal Article · Sun Feb 01 04:00:00 EST 1981 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00964838· OSTI ID:5405793

Peterson, R G; Baughman, S; Scheidler, D M

The simultaneous incorporation of (/sup 3/H)fucose and (1-/sup 14/C)leucine into normal rat sciatic nerve was examined using an in vitro incubation model. A linear rate of protein precursor uptake was found in purified myelin protein over 1/2-6 hr of incubation utilizing a supplemented medium containing amino acids. This model was then used to examine myelin protein synthesis in nerves undergoing degeneration at 1-4 days following a crush injury. Data showed a statistically significant decrease in the ratio of fucose to leucine at 2, 3, and 4 days of degeneration, which was the consequence of a significant increase in leucine uptake. These results, plus substantial protein recovery in axotomized nerves, are indicative of active synthesis of proteins that purify with myelin during early Wallerian degeneration.

• OSTI ID: 5405793
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 6:2; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
HEXOSES
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
LIPOPROTEINS
MAMMALS
MATHEMATICS
MEDICINE
MONOSACCHARIDES
MYELIN
NERVES
NERVOUS SYSTEM
NEUROLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHYSIOLOGY
PROTEINS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SCIATIC NERVE
STATISTICS
SYNTHESIS
TIME DEPENDENCE
TRITIUM COMPOUNDS
VERTEBRATES