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Altered metabolic incorporation of fucose and leucine into PNS myelin of 25-week-old diabetic (C57BL/Ks (db/db)) mice: effects of untreated diabetes on nerve metabolism

Journal Article · · Neurochem. Res.; (United States)
DOI:https://doi.org/10.1007/BF00965102· OSTI ID:5590285
Sciatic nerves of 25-week-old genetically diabetic (C57BL/Ks (db/db)) mice and their litter-mate controls were removed, and their metabolic incorporation of (/sup 3/H)fucose and (/sup 14/C)leucine into myelin was studied in vitro. Untreated diabetic animals showed significant increases (p less than 0.05) in the fucose/leucine incorporation into myelin when compared to values found for their litter-mates. These results correlated well with previous experiments performed on alloxan or streptozotocin-diabetic rats and thus show the in vitro incubation procedure to be a good indicator of altered metabolic conditions in peripheral nerves due to diabetes mellitus. The resulting ratio increases seen in diabetic animals is at variance with the decrease in ratios found in animals undergoing typical Wallerian degeneration. These results suggest that different metabolic processes operate in untreated diabetics than in normals or in those undergoing other degenerative nerve processes.
Research Organization:
Department of Anatomy, Indiana University School of Medicine, Indianapolis
OSTI ID:
5590285
Journal Information:
Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 8:4; ISSN NERED
Country of Publication:
United States
Language:
English