Altered glycosaminoglycan metabolism in injured arterial wall

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Exp. Mol. Pathol.; (United States)

DOI:https://doi.org/10.1016/0014-4800(85)90081-4· OSTI ID:5095713

Salisbury, B G; Hajjar, D P; Minick, C R

Glycosaminoglycans (GAG) are believed to be important in the pathogenesis of atherosclerosis. We have previously demonstrated that areas of injured aorta that have been re-endothelialized accumulate increased amounts of lipid and GAG when compared to areas remaining de-endothelialized. We have now examined the net incorporation of labeled precursors into the individual GAG present in both re-endothelialized and de-endothelialized areas of rabbit aorta. Aortic tissue was examined at 2-3 and 10-14 weeks after a denuding injury by incubating tissue minces with (/sup 3/H)glucosamine and sodium (/sup 35/S)sulfate for 24 hr. Following incubation, the aortic GAG were isolated and assayed for uronic acid concentration and radioactivity. Results indicate that the total GAG concentration was significantly greater in the re-endothelialized as compared to de-endothelialized areas. The concentration in uninjured aorta was 9.01. The difference between the injured tissues was attributable to increased concentrations of sulfated GAG. Hyaluronic acid and chondroitin sulfate were the most metabolically active of the GAG in either uninjured or injured aorta, together accounting for over 75% of the /sup 3/H label. The /sup 3/H specific radioactivities of the four GAG in the short-term, re-endothelialized subgroup were all increased nearly twice that found in uninjured and de-endothelialized tissues. With the exception of heparan sulfate, no significant differences were noted in the /sup 3/H specific radioactivities between the re-endothelialized and de-endothelialized areas in the long-term subgroup. These results indicate that, relative to adjacent areas of de-endothelialization, GAG preferentially accumulate in re-endothelialized areas even as early as 2-3 weeks following a denuding injury.

• OSTI ID: 5095713
• Journal Information: Exp. Mol. Pathol.; (United States), Journal Name: Exp. Mol. Pathol.; (United States) Vol. 3; ISSN EXMPA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
AORTA
ARTERIES
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHEMISTRY
CHONDROITIN
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ENDOTHELIUM
EVEN-ODD NUCLEI
GLUCOSAMINE
HEMATOLOGIC AGENTS
HEPARIN
HEXOSAMINES
HEXOSES
HYALURONIC ACID
HYDROGEN ISOTOPES
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLISM
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOGENESIS
POLYSACCHARIDES
RABBITS
RADIOISOTOPES
RECOVERY
REGENERATION
REPAIR
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES